The Peptide Reference
The Peptide Reference
References
Reference/Heterodimeric glycoprotein

HCG

Human Chorionic Gonadotropin · LH Receptor Agonist

Human clinical
research use only

HCG is a glycoprotein hormone naturally produced by the placenta during pregnancy that binds LH receptors to stimulate testosterone and estrogen biosynthesis. FDA-approved for cryptorchidism, hypogonadotropic hypogonadism, and ovulation induction.

Maintains testicular function during TRTPreserves fertility and prevents testicular atrophyStimulates endogenous testosterone productionInduces ovulation in women
01

Overview

HCG is a glycoprotein hormone naturally produced by the placenta during pregnancy that binds LH receptors to stimulate testosterone and estrogen biosynthesis. FDA-approved for cryptorchidism, hypogonadotropic hypogonadism, and ovulation induction.

Binds to LH receptors on Leydig cells in testes, stimulating testosterone production with a half-life of 24-36 hours, peak levels 6-12 hours post-injection, and 40-50% bioavailability via SubQ or IM routes.

Evidence profilederived from 5 references
A
Human clinical
Strongest design among the references on this page. Grade and effect size are separate facts and are never merged into one score.
02

Research indications

What the compound has been studied for, grouped by body system. Effect size is the reported magnitude where it was measured — not a recommendation.

Male Fertility3
TRT Adjunct

Maintains intratesticular testosterone at baseline during testosterone therapy, preventing atrophy and preserving fertility.

Large
Hypogonadotropic Hypogonadism

FDA-approved for secondary hypogonadism; combined with FSH for spermatogenesis induction.

Large
Post-Cycle Therapy

Restores testicular function after anabolic steroid cycles.

Moderate
Female Fertility1
Ovulation Induction

FDA-approved trigger for follicular maturation; 15-25% pregnancy rate per cycle.

Large
Pediatric1
Cryptorchidism

FDA-approved for prepubertal undescended testes not due to anatomical obstruction; ~25% success rate.

Small
i

A large effect in a weak study and a small effect in a strong one are different things. Effect size is never evidence of use in people.

Quick factsreference only
Class
Heterodimeric glycoprotein
Chain length
237 residues
Molecular weight
36700 Da
Half-life
~30 h
Typical dose
250-1500 IU (lower for TRT adjunct, higher for fertility)
Frequency
2-3 times weekly, or every other day for lower doses
Cycle length
Ongoing with TRT or 3-6 months for fertility protocols
Storage
Lyophilized: Room temperature. Reconstituted: 2-8°C, use within 30-60 days
03

Molecular data

Type
Heterodimeric glycoprotein
Molecular weight
36700 Da
Chain length
237 residues
Half-life
1800 min
04

Dosing reference

Doses reported in the literature and by suppliers. These are a record of what has been used in research — reference, never instruction.

ContextRouteAmountFrequency
TRT Adjunct (Low)SubQ/IM250-500 IUEvery other day
TRT Adjunct (Standard)SubQ/IM500-1000 IUTwice weekly
HCG MonotherapyIM1500-2000 IU2-3x weekly
Fertility (with FSH)IM1500-2000 IU2-3x weekly
Cryptorchidism (Pediatric)IM1000-5000 IU2-3x weekly for 3-4 weeks
Ovulation Trigger (Female)IM/SubQ5000-10,000 IUSingle dose
PCT ProtocolSubQ/IM1000-1500 IUEvery other day for 2-3 weeks
05

Interactions

Testosterone

Commonly combined in TRT to maintain testicular function and preserve fertility.

synergistic
Clomiphene (Clomid)

Use sequentially; both affect HPG axis.

monitor
Aromatase Inhibitors

Commonly combined; HCG increases intratesticular aromatase activity.

compatible
GnRH Analogs

Monitor combination effects on gonadotropin axis.

monitor
FSH (Follitropin)

Synergistic for fertility; 70-90% spermatogenesis induction rate.

synergistic
Kisspeptin

Complementary mechanisms for HPG axis stimulation.

compatible
Thyroid Hormones

HCG has weak TSH-like activity; monitor thyroid function.

monitor
Metformin

Protective combination for metabolic health.

compatible
06

Quality checklist

  • White to off-white lyophilized powder or cake in sealed vial
  • Completely clear solution after reconstitution
  • Proper labeling: Pregnyl, Novarel (urinary), Ovidrel (recombinant)
  • Clear expiration and lot number
  • Cold chain compliance (recombinant requires refrigeration throughout)
  • !Generic/compounding pharmacy products - quality varies
  • !Ensure compounding pharmacy is accredited
  • ×Cloudiness, discoloration, or floating particles indicates degradation
  • ×Compromised vial seal or expired product
07

What to expect

Day 1-3Cellular-level action begins; no immediate noticeable effects
Week 1-2Testosterone increase detectable on labs; possible mood/energy improvement
Week 2-4Testicular fullness/size improvement noticeable; improved well-being
Week 4-8Stable testosterone levels; fertility parameters beginning to improve
Month 2-3Sperm count improvements if used for fertility; sustained testicular function
Long-termMaintained testicular size and function with ongoing use
08

Safety

Commonly reported4
  • Gynecomastia (breast tenderness/swelling) due to increased estrogen
  • Headaches, irritability, and mood swings (especially initially)
  • Fluid retention and edema
  • Potential antibody formation with long-term use
Stop and seek advice8
  • Signs of gynecomastia (breast tenderness, swelling, nipple sensitivity)
  • Severe or persistent headaches
  • Signs of blood clots (leg swelling/pain, shortness of breath, chest pain)
  • Allergic reactions (rash, hives, difficulty breathing, facial swelling)
  • Severe abdominal pain or bloating in women (possible OHSS)
  • Testicular pain or swelling beyond normal
  • Significant mood changes (depression, aggression, severe irritability)
  • Vision changes
Contraindications3
  • Hormone-sensitive cancers (prostate, breast)
  • Pregnancy (except as prescribed)
  • Precocious puberty risk in children
09

FAQ

How much HCG is needed to maintain fertility during testosterone replacement therapy?

A landmark clinical study found 250 IU of HCG every other day maintained intratesticular testosterone within 7% of baseline during testosterone therapy, preserving fertility. This low dose is far less than other protocols suggesting HCG is remarkably potent—even minimal doses maintain testicular function when properly timed.

Why does HCG cause gynecomastia if it just stimulates testosterone?

HCG stimulates testosterone production, but testes also express aromatase enzyme that converts testosterone to estrogen. The increased testosterone availability combined with enhanced intratesticular aromatase activity results in elevated estrogen, causing breast tenderness and gynecomastia. Aromatase inhibitors help prevent this side effect.

Can antibodies to HCG develop with long-term use and reduce effectiveness?

Potential antibody formation to HCG with extended use is a theoretical concern, though clinical significance remains unclear. Some users report diminishing HCG effectiveness after months of continuous use. Cycling HCG with breaks or rotation to GnRH analogs may prevent tolerance development.

What's the success rate of HCG for treating cryptorchidism (undescended testes)?

Meta-analysis of HCG in cryptorchidism shows approximately 24% success rate—modest but clinically relevant for select cases. Success is higher for bilateral versus unilateral cryptorchidism. This low success rate led to surgery becoming the standard treatment for most cases, though HCG remains an initial option.

10

References

  1. 1
    Low-Dose Human Chorionic Gonadotropin Maintains Intratesticular Testosterone in Normal Men with Testosterone-Induced Gonadotropin Suppression
    Coviello AD, Matsumoto AM, Bremner WJ, et al. · Journal of Clinical Endocrinology & Metabolism · 2005

    29 men randomized to testosterone enanthate plus 125, 250, or 500 IU HCG every other day. 250 IU maintained intratesticular testosterone within 7% of baseline, preserving fertility potential during TRT.

  2. 2
    The Effectiveness of hCG and LHRH in Boys with Cryptorchidism: A Meta-Analysis of Randomized Controlled Trials
    Defined a, et al. · Asian Journal of Andrology · 2016

    Meta-analysis of 13 studies with 872 boys and 1,174 undescended testes. Overall HCG success rate of 24%. Significant effect on bilateral but not unilateral cryptorchidism.

    meta-analysisPubMed 27050251
  3. 3
    Fertility Induction in Hypogonadotropic Hypogonadal Men
    Rastrelli G, Corona G, Mannucci E, Maggi M · Endocrine Reviews · 2018

    Combined HCG and FSH therapy induces spermatogenesis in 86% (95% CI 82-91%) of men with hypogonadotropic hypogonadism. HCG alone achieves 40% (95% CI 25-56%).

  4. 4
    Efficacy and Safety of Human Chorionic Gonadotropin for Treatment of Cryptorchidism: A Meta-Analysis of Randomised Controlled Trials
    Defined a, et al. · Journal of Pediatric Surgery · 2018

    Confirmed ~25% success rate for HCG in cryptorchidism treatment. All side effects were transitory and not severe.

    meta-analysisPubMed 29655188
  5. 5
    Human Chorionic Gonadotropin Monotherapy for the Treatment of Hypogonadal Symptoms in Men with Total Testosterone >300 ng/dL
    Alder NJ, Waguih WI, et al. · International Journal of Impotence Research · 2019

    HCG monotherapy safe and efficacious for hypogonadal symptoms. Erectile dysfunction improved in 86% (19/22), libido in 80% (20/25). No thromboembolic events.

For research use only. Nothing on this page is medical advice, and no number here is a recommendation to dose.