The Peptide Reference
The Peptide Reference
References
Reference/Triple GLP-1/GIP/glucagon agonist

Retatrutide

Triple GLP-1/GIP/Glucagon Agonist · Weight Loss & Diabetes

Human clinical
research use only

Novel triple hormone receptor agonist targeting GLP-1, GIP, and glucagon receptors. Phase II trials demonstrated 24.2% weight loss at 48 weeks—the highest recorded for obesity medications.

Superior weight loss (24.2% at 48 weeks)Improved glycemic control (HbA1c reduction up to 2.16%)Enhanced cardiovascular benefitsHepatic fat reduction (up to 82%)
01

Overview

Novel triple hormone receptor agonist targeting GLP-1, GIP, and glucagon receptors. Phase II trials demonstrated 24.2% weight loss at 48 weeks—the highest recorded for obesity medications.

Activates GLP-1 for appetite suppression, GIP for insulin sensitivity, and glucagon for increased energy expenditure and hepatic fat oxidation.

Evidence profilederived from 6 references
A
Human clinical
Strongest design among the references on this page. Grade and effect size are separate facts and are never merged into one score.
02

Research indications

What the compound has been studied for, grouped by body system. Effect size is the reported magnitude where it was measured — not a recommendation.

Weight Loss3
Superior Weight Reduction

Clinical trials show 17.5% weight loss at 24 weeks and 24.2% at 48 weeks.

Large
Sustained Weight Management

Continuous weight loss with no plateau at 48 weeks suggests greater long-term potential.

Large
Triple Mechanism

Addresses obesity through appetite suppression, energy expenditure, and metabolic efficiency.

Large
Type 2 Diabetes3
Superior Glycemic Control

HbA1c reductions up to 2.16% with 82% achieving target levels below 6.5%.

Large
Glucose-Dependent Regulation

Balanced glycemic control with minimal hypoglycemia risk.

Moderate
Insulin Sensitivity

Marked improvements in sensitivity with potential for reduced exogenous insulin requirements.

Moderate
Cardiovascular/Metabolic3
Lipid Improvement

Non-HDL cholesterol reductions up to 26.9%, triglyceride reductions up to 40.6%.

Moderate
Blood Pressure Optimization

Consistent decreases in systolic and diastolic blood pressure across trials.

Moderate
Hepatic Fat Reduction

Up to 82% reduction in liver fat with normalization in 90% of participants.

Large
i

A large effect in a weak study and a small effect in a strong one are different things. Effect size is never evidence of use in people.

Quick factsreference only
Class
Triple GLP-1/GIP/glucagon agonist
Chain length
39 residues
Molecular weight
4731.33 Da
Half-life
~144 h
Typical dose
0.5mg starting, titrate up to 8-12mg weekly
Frequency
Once weekly (same day each week)
Cycle length
Continuous therapy as prescribed
Storage
Reconstituted: 2-8°C, use within 28 days
03

Molecular data

Type
Triple GLP-1/GIP/glucagon agonist
Molecular weight
4731.33 Da
Chain length
39 residues
Half-life
8640 min
04

Dosing reference

Doses reported in the literature and by suppliers. These are a record of what has been used in research — reference, never instruction.

ContextRouteAmountFrequency
Starting Dose (Week 1-4)SubQ0.5mgOnce weekly
Low Maintenance (Week 4-8)SubQ1mgOnce weekly
Escalation (Week 8-12)SubQ2mgOnce weekly
Moderate (Week 12-16)SubQ4mgOnce weekly
Advanced (Week 16-20)SubQ8mgOnce weekly
Maximum Efficacy (Week 20+)SubQ12mgOnce weekly
05

Interactions

Tirzepatide

Do not combine with other dual/triple agonists—risk of severe hypoglycemia and excessive GI effects.

avoid
Semaglutide

Do not combine—overlapping GLP-1 agonist mechanisms increase severe hypoglycemia risk.

avoid
Cagrilintide

Both cause significant GI effects. Not recommended without specialist supervision.

monitor
Insulin

May significantly reduce insulin requirements. Monitor blood glucose and adjust insulin doses accordingly.

monitor
Metformin

Safe combination tested in clinical trials. Different mechanisms work complementarily for glucose control.

compatible
SGLT2 Inhibitors

Clinical trials included SGLT2 inhibitors with no safety concerns.

compatible
BPC-157

Safe combination; BPC-157 may provide GI protective benefits during retatrutide use.

compatible
Oral Contraceptives

Space oral contraceptives by 1 hour before retatrutide due to delayed gastric emptying.

monitor
06

Quality checklist

  • Pharmaceutical-grade white powder with uniform texture
  • Proper cold chain maintenance (2-8°C refrigeration)
  • Clear, colorless reconstituted solution without particles
  • Stable extended half-life effects (consistent appetite suppression between doses)
  • !Source verification critical—counterfeit versions circulate due to investigational status
  • ×Rapid tolerance or effectiveness loss suggests degraded or counterfeit product
  • ×Unusual side effect profile may indicate contamination
07

What to expect

Week 1-2Initial appetite suppression and mild GI effects as body adapts to triple hormone activation
Week 2-4Noticeable food cravings reduction and portion size decrease; early weight loss (2-5%)
Week 4-8Significant appetite control and steady weight loss (5-10%); improved glucose control
Week 8-16Substantial weight reduction (10-18%) with enhanced energy expenditure
Week 16-24Major weight loss milestone (15-22%) with cardiovascular benefits and liver fat reduction
Week 24-48Maximum clinical efficacy (20-24.2%) with comprehensive metabolic improvements
08

Safety

Commonly reported4
  • Gastrointestinal effects (nausea, vomiting, diarrhea)—typically mild to moderate
  • Heart rate increases—common especially in first 24 weeks
  • Appetite suppression
  • Mild dehydration
Stop and seek advice5
  • Severe persistent nausea or vomiting preventing adequate nutrition
  • Signs of pancreatitis: severe abdominal pain radiating to back
  • Severe hypoglycemia symptoms: confusion, dizziness, sweating
  • Excessive weight loss (>3 lbs per week consistently or >25% total body weight)
  • Gallbladder problems: severe right upper abdominal pain
Contraindications3
  • Personal or family history of medullary thyroid carcinoma
  • MEN2 syndrome
  • Severe renal impairment
09

FAQ

What makes retatrutide's 24.2% weight loss so much higher than semaglutide's 15-20%?

Retatrutide is a triple agonist activating GLP-1, GIP, and glucagon receptors simultaneously. GLP-1 suppresses appetite, GIP improves insulin sensitivity, and glucagon increases energy expenditure and fat oxidation. Semaglutide only activates GLP-1. The triple mechanism addresses obesity through three distinct pathways, explaining the superior results.

Does retatrutide actually reduce liver fat, or just overall fat loss?

It specifically targets liver fat. Clinical trials showed up to 82% liver fat reduction and complete normalization in 90% of participants at 24 weeks. This isn't just general weight loss—it's preferential hepatic fat oxidation, making retatrutide potentially valuable for NAFLD/MASH, not just obesity.

Why is retatrutide more likely to cause gastrointestinal side effects?

Triple agonism means triple GI effects. GLP-1, GIP, and glucagon all slow gastric emptying and affect GI motility. Combining three mechanisms means more nausea, diarrhea, and vomiting, especially during dose escalation. However, most people tolerate it by week 4-8 as their body adapts.

Could I eventually stop taking retatrutide, or do I need it forever?

Unknown. Phase 2 trials ran 48 weeks—long enough to achieve 24% weight loss but not long enough to study discontinuation. Clinical experience with semaglutide suggests weight returns if stopped. Phase 3 TRIUMPH trials (results expected 2026) should provide data on maintenance therapy vs. indefinite use.

10

References

  1. 1
    Triple-Hormone-Receptor Agonist Retatrutide for Obesity — A Phase 2 Trial
    Jastreboff, A.M., et al. · New England Journal of Medicine · 2023

    Landmark phase 2 RCT showing dose-dependent weight loss: 24.2% at 48 weeks with 12mg dose — the highest recorded for any obesity medication at the time. Weight reduction of ≥15% achieved in 83% of 12mg recipients.

  2. 2
    Retatrutide, a GIP, GLP-1 and glucagon receptor agonist, for people with type 2 diabetes: a phase 2 trial
    Rosenstock, J., et al. · The Lancet · 2023

    Phase 2 trial in type 2 diabetes demonstrating clinically meaningful HbA1c reductions and robust weight loss of up to 16.9% at 36 weeks, with safety profile consistent with GLP-1 receptor agonists.

  3. 3
    Triple hormone receptor agonist retatrutide for metabolic dysfunction-associated steatotic liver disease: a randomized phase 2a trial
    Sanyal, A.J., et al. · Nature Medicine · 2024

    Dose-dependent liver fat reduction: 82.4% at 12mg dose. Normal liver fat (<5%) achieved in 86% of 12mg recipients vs 0% placebo at 24 weeks. Improvements linked to changes in body weight, abdominal fat, and insulin sensitivity.

  4. 4
    Structural insights into the triple agonism at GLP-1R, GIPR and GCGR manifested by retatrutide
    Li, W., et al. · Cell Discovery · 2024

    Cryo-EM structures reveal how retatrutide simultaneously activates GLP-1R, GIPR, and GCGR through distinct receptor-binding modes, explaining the molecular basis for its triple agonist activity and superior clinical efficacy.

  5. 5
    TRIUMPH registrational clinical trials: Rationale and design
    Aronne, L.J., et al. · Obesity · 2025

    Phase 3 TRIUMPH program design: four multicenter, randomized, double-blind studies assessing weekly retatrutide for obesity, obstructive sleep apnea, knee osteoarthritis, and weight management in cardiovascular disease populations.

  6. 6
    Effects of retatrutide on body composition in people with type 2 diabetes: a phase 2 substudy
    Rosenstock, J., et al. · The Lancet Diabetes & Endocrinology · 2025

    Substudy demonstrating significant total body fat mass reduction with retatrutide vs placebo and dulaglutide. Proportion of lean mass loss to total weight loss was similar to other obesity treatments.

For research use only. Nothing on this page is medical advice, and no number here is a recommendation to dose.