The Peptide Reference
The Peptide Reference
References
Reference/Modified hGH C-terminal fragment

AOD-9604

Modified hGH Fragment · Fat Loss Peptide

Animal in vivo
research use only

AOD-9604 is a modified fragment of human growth hormone (amino acids 176-191) that stimulates lipolysis and inhibits lipogenesis without the side effects of full growth hormone. Unlike full GH, it does not increase IGF-1, affect glucose metabolism, or cause insulin resistance.

Targets fat loss through selective lipolysisAvoids growth hormone side effectsNo effect on blood glucose or insulinDoes not increase IGF-1 levels
01

Overview

AOD-9604 is a modified fragment of human growth hormone (amino acids 176-191) that stimulates lipolysis and inhibits lipogenesis without the side effects of full growth hormone. Unlike full GH, it does not increase IGF-1, affect glucose metabolism, or cause insulin resistance.

Stimulates lipolysis and inhibits lipogenesis through beta-3 adrenergic receptor upregulation, without binding to the GH receptor. Does not affect glucose metabolism or increase IGF-1 levels.

Evidence profilederived from 5 references
C
Animal in vivo
Strongest design among the references on this page. Grade and effect size are separate facts and are never merged into one score.
02

Research indications

What the compound has been studied for, grouped by body system. Effect size is the reported magnitude where it was measured — not a recommendation.

Fat Loss3
Lipolysis Enhancement

Increases fat oxidation, plasma glycerol levels, and reduces body weight in obese models without affecting glucose metabolism.

Large
Lipogenesis Inhibition

Reduces fat synthesis and storage in adipose tissue.

Moderate
Metabolic Safety

Does not cause hyperglycemia, reduce insulin secretion, or increase insulin resistance.

Moderate
Joint Health2
Cartilage Repair

Enhanced cartilage regeneration when combined with hyaluronic acid in osteoarthritis models.

Moderate
Osteoarthritis Support

Synergistic effects with hyaluronic acid for joint health.

Small
i

A large effect in a weak study and a small effect in a strong one are different things. Effect size is never evidence of use in people.

Quick factsreference only
Class
Modified hGH C-terminal fragment
Chain length
17 residues
Molecular weight
1815.1 Da
Half-life
~4 min
Typical dose
250-500mcg
Frequency
Once daily
Cycle length
8-12 weeks
Storage
Lyophilized: room temp or freezer long-term. Reconstituted: 2-8°C for 28 days
03

Molecular data

Type
Modified hGH C-terminal fragment
Molecular weight
1815.1 Da
Chain length
17 residues
Half-life
4 min
Primary sequenceN → C · 16 residues
H₂N–
YTyr1
LLeu2
RArg3
IIle4
VVal5
QGln6
CCys7
RArg8
SSer9
VVal10
EGlu11
GGly12
SSer13
CCys14
GGly15
FPhe16
–OH
YLRIVQCRSVEGSCGF
NonpolarPolarAcidicBasic
04

Dosing reference

Doses reported in the literature and by suppliers. These are a record of what has been used in research — reference, never instruction.

ContextRouteAmountFrequency
Fat loss - StandardSubQ250-300mcgOnce daily (morning, fasted)
Enhanced fat lossSubQ400-500mcgOnce daily (morning, fasted)
Joint supportSubQ250mcgOnce daily
Conservative startSubQ200mcgOnce daily
05

Interactions

Hyaluronic Acid

Enhanced cartilage repair when combined for joint health.

synergistic
BPC-157

Different mechanisms; sometimes combined for comprehensive healing.

compatible
CJC-1295

AOD-9604 doesn't affect GH axis; can be used together.

compatible
Ipamorelin

Different mechanisms; no known interactions.

compatible
Semaglutide

Complementary weight loss mechanisms through different pathways.

compatible
Insulin

AOD-9604 doesn't affect glucose metabolism unlike full hGH.

compatible
06

Quality checklist

  • White lyophilized powder with uniform appearance
  • Crystal clear solution without particles after reconstitution
  • Certificate of Analysis showing >98% purity by HPLC
  • Proper cold-chain shipping
  • !Not FDA approved as a therapeutic drug - research chemical only
  • !WADA prohibited substance
  • ×Discolored or clumped powder (yellow indicates degradation)
  • ×Cloudy solution after reconstitution
  • ×Collapsed or moisture-damaged appearance
07

What to expect

Week 1-2Minimal noticeable effects; compound accumulating
Week 3-4Subtle body composition changes may begin
Week 5-8More noticeable fat loss, especially with diet and exercise
Week 8-12Continued fat reduction and improved body composition
08

Safety

Commonly reported3
  • Generally well-tolerated in clinical trials
  • Mild injection site reactions possible
  • No reported effects on blood glucose or insulin
Stop and seek advice4
  • Severe injection site reactions or infection signs
  • Unusual swelling or persistent redness
  • Allergic reactions (rare)
  • Any concerning or unexpected symptoms
Contraindications2
  • Pregnancy or breastfeeding
  • WADA prohibited - athletes subject to testing must avoid
09

FAQ

Does AOD-9604 cause blood sugar problems like full human growth hormone?

No. AOD-9604 is a modified hGH fragment that stimulates lipolysis without binding to the GH receptor or affecting glucose metabolism. Unlike full GH which causes hyperglycemia and insulin resistance, AOD-9604 does not increase IGF-1 levels or disrupt glucose homeostasis, making it metabolically safer.

How quickly will I see fat loss with AOD-9604?

Week 1-2 shows minimal noticeable effects as compound accumulates. Week 3-4 brings subtle body composition changes. Week 5-8 produces more noticeable fat loss especially with diet and exercise. Week 8-12 shows continued fat reduction and improved body composition. Total timeline is slower than GLP-1 agonists.

Can AOD-9604 be stacked with semaglutide or other weight loss peptides?

Yes. AOD-9604's lipolytic mechanism complements semaglutide's appetite suppression through completely different pathways. However, combination data is limited. Both are safe separately; combining requires monitoring for additive dehydration/GI effects common with semaglutide.

Is there any cartilage repair evidence for AOD-9604 in humans?

Animal studies show AOD-9604 combined with hyaluronic acid enhanced cartilage regeneration in osteoarthritis models. However, this is preclinical; human cartilage repair data doesn't exist. Joint health claims should be considered theoretical unless based on clinical trial data.

10

References

  1. 1
    Effects of oral administration of a synthetic fragment of human growth hormone on lipid metabolism
    Heffernan MA, Heffernan MJ, Jiang WJ, Thorburn AW, Ng FM · American Journal of Physiology - Endocrinology and Metabolism · 2000

    Oral treatment of ob/ob mice with AOD-9401 for 30 days significantly reduced body weight gain and altered lipogenic and lipolytic activity in adipose tissue.

  2. 2
    Metabolic studies of a synthetic lipolytic domain (AOD9604) of human growth hormone
    Ng FM, Jiang WJ, Gianello R, Pitt C, Heffernan M · Hormone Research · 2000

    Characterized metabolic properties of AOD-9604 as a synthetic lipolytic domain of hGH with antilipogenic activity that does not interact with the GH receptor.

  3. 3
    Increase of fat oxidation and weight loss in obese mice caused by chronic treatment with human growth hormone or a modified C-terminal fragment
    Heffernan MA, Thorburn AW, Fam B, Summers R, Conway-Campbell B, Waters MJ, Ng FM · International Journal of Obesity · 2001

    Both hGH and AOD-9604 reduced body weight gain, increased fat oxidation, and stimulated lipolysis in obese ob/ob mice after 14 days chronic IP administration, without affecting glucose metabolism.

  4. 4
    The effects of human GH and its lipolytic fragment (AOD9604) on lipid metabolism following chronic treatment in obese mice and beta(3)-AR knock-out mice
    Heffernan MA, Jiang WJ, Thorburn AW, Ng FM · Endocrinology · 2001

    Both hGH and AOD-9604 increased repressed beta-3 adrenergic receptor RNA expression in obese mice to levels comparable with lean mice. Effects abolished in beta-3-AR knockout mice, confirming pathway involvement.

  5. 5
    Effect of Intra-articular Injection of AOD9604 with or without Hyaluronic Acid in Rabbit Osteoarthritis Model
    Kwon DR, Park GY, Lee SU · Annals of Clinical and Laboratory Science · 2015

    Intra-articular AOD-9604 injections enhanced cartilage regeneration in collagenase-induced osteoarthritis rabbit model. Combined AOD-9604 and hyaluronic acid injections were more effective than either alone.

For research use only. Nothing on this page is medical advice, and no number here is a recommendation to dose.